Table 1 Strains, plasmids and primers used in this study
From: Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate
Primers or plasmids | Description | Source or reference |
|---|---|---|
Bacterial strains | ||
B. abortus S2308 | Wild type strain; smooth phenotype | ATCC |
RB51 | Vaccine strain; rough phenotype | This study |
∆22915 | BAB_RS22915 gene deletion mutant strain; smooth phenotype | This study |
Escherichia coli (DH5α) | F− φ80lacZ∆M15∆(lacZYA-argF)U169 recA1 endA1 hsdR17(r −k , m +k ) phoA supE44 thi-1 gyrA96 relA1 λ− | Tiangen |
Plasmids | ||
pSC | AmpR; pUC19 plasmid containing SacB gene | [22] |
Primers | ||
BAB_RS22915 UF | GCTCTAGACGTATATTCATCATCCGCAG (XbaI underlined) | This study |
BAB_RS22915 UR | TGAGTTGATCCTGCGTCAGACTGAGGCGATAATCTTCATG | This study |
BAB_RS22915 DF | CATGAAGATTATCGCCTCAGTCTGACGCAGGATCAACTCA | This study |
BAB_RS22915 DR | GCTCTAGAGACATTGGAGGTGATTGCC (XbaI underlined) | This study |
BAB_RS22915 FF | GCCACCCAACTTAGCGTGAG | This study |
BAB_RS22915 FR | AAGTGGCGGCACCAAGAG | This study |
BAB_RS22915 OF | GATGGCAAGGTCGATCTG | This study |
BAB_RS22915 OR | GCCTGTCGAGAAGTTCCTG | This study |
RT-22910 F | AAAGCACCGTTTTGCTCATC | This study |
RT-22910 R | GCCAGACGGTTCATGTAGTG | This study |
RT-22920 F | CCTCATCTGGAAAGTGCTGC | This study |
RT-22920 R | CGAGAAAGAGTCCAAGCGTG | This study |
RT-22915 F | ATAATGCCGTCAACATGCCG | This study |
RT-22915 R | GGAAATGAGGCGCTTGGAAA | This study |
RT-GAPDH F | GACATTCAGGTCGTCGCCATCA | [23] |
RT-GAPDH R | TCTTCCTTCCACGGCAGTTCGG | [23] |