Figure 3
From: Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate
Comparison of the persistence of mutant strain ∆22915, wild-type strain S2308 and vaccine strain RB51. Five female BALB/c mice were IP-inoculated with ∆22915, S2308 or RB51 at 1 × 105 CFU/mouse. A Bacterial loads in spleens were determined at 2, 4, 6, 9 and 12 weeks post infection. Persistence of ∆22915 in mice was significantly decreased compared to that of strain S2308 at all time points investigated. Vaccine strain RB51 was cleared at 9 wpi. B There was no significant difference in spleen weights among the uninfected mice, or mice infected with mutant strain ∆22915, or vaccine strain RB51. Spleen weights from S2308-infected mice were significantly increased. Data were presented as mean ± SD (n = 5), and analyzed with two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.