Figure 8
From: Advanced biosensors for detection of pathogens related to livestock and poultry
Cartoon adapted from [94].
Fluorescent detection of viral nucleic acids on magnetic beads. Schematic presentation of the FRET-based magnetic biosensor for Orbiviruses detection. (1) A nucleic acid probe labeled with florescent dye, biotin and dark quencher hybridize with the complementary probe, (2) the fluorescent dye is separate from the quencher in each PCR cycle, and starts to produce light, (3) the fluorescent dye bind to streptavidin-coated magnetic beads via biotin tag, (4) about 1000 fluorescent-labeled probes bind to a single beads giving an increased fluorescent signal.