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Figure 1 | Veterinary Research

Figure 1

From: Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture

Figure 1

CEV load and replication in tissues of common carp affected by KSD. Carp edema virus load (A) was measured by qPCR as copy numbers of virus specific DNA. As a surrogate for CEV replication, the expression of the gene encoding the CEV core protein P4a (CEV P4a) was determined (B). The data on virus load and expression of viral mRNA are shown as box plots indicating the range of 25–75% in the box (±minimum and maximum values) of genome copies in 250 ng of isolated DNA from n = 4 fish (virus load), or as the copy number of mRNA encoding the viral P4a gene normalised against 100 000 copies of the carp 40S ribosomal protein S11. Symbols “+” and “□” indicate mean and median, respectively. Different letters indicate significant differences at p ≤ 0.05 between the carp tissues.

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Veterinary Research

ISSN: 1297-9716