Figure 2

Quantification and differentiation of Salmonella by qPCR. Bacterial loads of fecal samples collected from birds infected with Salmonella Typhimurium only (black line) or co-infected with Salmonella Typhimurium (red line) and Salmonella Mbandaka (red hashed line) were quantified using a serovar specific qPCR. Salmonella Typhimurium was detected using a primers designed to the TSR3 gene while Salmonella Mbandaka was detected using the dhrfV gene. Data are presented as mean log₁₀ CFU/gram feces ± SEM. The amount of bacteria varied significantly over time (p < 0.01).