Figure 6
RBS entry into SB cells is dependent on cytoskeleton. SB cells were treated with chemical inhibitors of CytoD (A), wiskostatin (B), and nocodazole (C) respectively at the indicated doses for 1 h. VLP entry assays, IFA, fluorescent microscopy, and ELISA were performed. RBS (green) in cells were visualized by IFA and cell nuclei (blue) were stained by DAPI. The entry efficiency was examined by ELISA and the data shown are the mean ± SD of the results from three independent experiments. The bars indicate 25 μm. **, p < 0.01.