Figure 4

Entry of RBS is independent of macropinocytosis. A Inhibitors of macropinocytosis were used individually. B Inhibitors of macropinocytosis and CME were used simultaneously. All inhibitors of macropinocytosis (40 μM) including Rottlerin (R), IPA-3 (I), ML-7 (M), and NSC23766 (N), and CME inhibitor (7 μM) CPZ (C) were used, alone or overlay as indicated +, to treat SB cells for 1 h. Two hours of VLP entry assays with the inhibitors and the following ELISA were performed. RBS entry without inhibitor was served as the untreated control (+). *, p < 0.05, **, p < 0.01.