Figure 2

RBS entry depends on CME and dynamin. A–D CPZ and dynasore reduce the entry and trafficking of RBS to the perinuclear region of SB cells. SB cells were pretreated with CPZ or dynasore at the indicated doses for 1 h, or left untreated, then incubated with RBS for 2 h, and fixed for IFA (A, B) or collected for ELISA (C, D). In the untreated or mock-infected controls, cells were subjected to no inhibitor treatment or no RBS incubation respectively. RBS (green) in cells were visualized by IFA and cell nuclei (blue) were stained by DAPI. The entry efficiency was examined by ELISA and the data shown are the mean ± SD of the results from three independent experiments. E, F Inhibition of sbCH and sbCL by RNAi reduced the entry of RBS. siRNA specific to sbCH (siCH), sbCL (siCL) and siRNA with irrelevant sequence (siIR) were transfected to SB cells 48 h prior to VLP entry assay. After the entry assay, cells were fixed for IFA (E) or collected for ELISA (F). The bars in (A, B, E) indicate 25 μm. *, p < 0.05, **, p < 0.01.