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Figure 5 | Veterinary Research

Figure 5

From: Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae

Figure 5

Production of IL-17A by non-CD4 + cells and γδ T cells in the lung. Cells isolated from lung tissue were incubated overnight with APP-CCE, medium or PMA/Ionomycin and subsequently analyzed for CD4, TCR-γδ and IL-17A expression by FCM. Living lymphocytes excluding CD4+ T cells (not shown; see Additional file 1) were gated and further analyzed for expression of IL-17A and TCR-γδ. Data from the same animals as in Figure 2 are shown. Approximately 1 × 106 (APP and medium) and 2 × 105 (PMA/Ionomycin) cells are shown in the contour plots. Arrow heads are introduced in the main text.

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