Figure 3
Expression of CD8α by IL-17A + CD4 + T cells in the lung. Cells isolated from lung tissue were incubated overnight with APP crude capsular extract (APP-CCE), medium or PMA/Ionomycin and subsequently analyzed for CD4, CD8α and IL-17A expression by FCM. Living CD4+ T cells were gated (not shown; see Additional file 1) and subsequently investigated for expression of CD8α and IL-17A. Data from the same animals as in Figure 2 are shown. Approximately 1 × 105 (APP and medium) and 5 × 104 (PMA/Ionomycin) CD4+ T cells are shown in the contour plots.