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Table 2 Replication and contact transmission of HD-160A virus in guinea pigs

From: The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs

Virus

Replication in guinea pigs

Contact transmission in guinea pigs

Virus titers determined on day 3 post inoculation in

Seroconversion: positive/total

Seroconversion: positive/total (HI titers)

Nasal wash (log10EID50 ± SD /mL)

Organ (log10EID50 ± SD /g)

Lung

Trachea

Spleen

Kidney

Brain

Inoculated

Contact

HD-160A

4.5 ± 1.2

2.5 ± 0.2

0.8 ± 0.1

–a

–

–

2/2

3/3(40,80,40)

2/3(0,20,20)

HD/05

–

1.2 ± 0.2

–

–

–

–

0/2

0/3

0/3

  1. Groups of four guinea pigs were challenged intranasally with 106 EID50 of either test viruses in a volume of 300 μL (150 μL per nostril), of which two animals from each group were euthanized on day 3 pi. Tissue samples involving nasal wash, lung, trachea, spleen, kidney and brain were collected for virus titration in eggs. Sera from the remaining two animals were collected at the end of the two-week observation period, and pretreated overnight with the receptor-destroying enzyme of Vibrio cholerae.
  2. aVirus was not recovered from the undiluted tissue sample.
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Veterinary Research

ISSN: 1297-9716