The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs

Gu, Min; Li, Qunhui; Gao, Ruyi; He, Dongchang; Xu, Yunpeng; Xu, Haixu; Xu, Lijun; Wang, Xiaoquan; Hu, Jiao; Liu, Xiaowen; Hu, Shunlin; Peng, Daxin; Jiao, Xinan; Liu, Xiufan

doi:10.1186/s13567-017-0410-0

Veterinary Research

Table 1 HA amino acid variations between A/mallard/Huadong/S/2005(H5N1) and the H5NX clade 2.3.4.4 reassortant viruses

From: The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs

Virus	HA sequence ID in public database	Reference	Clade	Receptor binding specificity	Amino acid variation in HA^a
Virus	HA sequence ID in public database	Reference	Clade	Receptor binding specificity	90	160	222	227	244	266
A/mallard/Huadong/S/2005(H5N1)	EU195392^b	[17]	2.3.4	α-2,3	K	T	K	S	N	A
A/duck/Jiangsu/k1203/2010(H5N8)	JQ973694^b	[17]	2.3.4.4^d	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Shandong/Q1/2013(H5N8)	KM504101^b	[17]	2.3.4.4^d	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Eastern China/1111/2011(H5N2)	JQ041401^b	[17]	2.3.4.4^d	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Eastern China/1112/2011(H5N2)	JQ041402^b	[17]	2.3.4.4^d	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Eastern China/008/2008(H5N5)	GU727653^b	[18]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Eastern China/031/2009(H5N5)	GU727661^b	[18]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Shandong/k1204/2009(H5N5)	JQ973670^b	[18]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Guangdong/k0103/2010(H5N5)	JQ973686^b	[18]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/quail/Jiangsu/k0104/2010(H5N5)	JQ973678^b	[18]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Jiangsu/QD5/2014(H5N8)	KT221066^b	[19]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Shandong/WFSG1/2014(H5N8)	KT221074^b	[19]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Yangzhou/0420/2014(H5N8)	KT221082^b	[19]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Eastern China/CZ/2013(H5N8)	KX013029^b	[20]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Eastern China/JY/2014(H5N8)	KX013021^b	[20]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Eastern China/S0513/2013(H5N6)	KP732638^b	[21]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Eastern China/S0711/2014(H5N6)	KP732641^b	[21]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/duck/Eastern China/S0908/2014(H5N6)	KP732643^b	[21]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/goose/Eastern China/S0322/2014(H5N6)	KP732644^b	[21]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/migratory waterfowl/Hubei/Chenhu1347/2014(H5N6)	EPI_ISL_234377^c	[22]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T
A/Anascrecca/Hubei/Chenhu1623-5/2014(H5N6)	EPI_ISL_179644^c	[22]	2.3.4.4	α-2,3+α-2,6	R ^e	A	Q	R	H	T

^aH3 numbering, amino acids were in single-letter abbreviation. ‘‘K’’ is lysine, ‘‘R’’ isargnine, ‘‘S’’ isserine, ‘‘T’’ is threonine, ‘‘A’’ is alanine, ‘‘Q’’ is glutamine, ‘‘N’’ is asparagine, and ‘‘H’’ is histidine.
^bHA sequence ID in GenBank.
^cHA sequence ID in GISAID.
^dAlthough the H5 subtype virus was initially clustered into clade 2.3.4.6 when published in Ref. [17], the provisional clade 2.3.4.6 designation has already been replaced by the unified classification of clade 2.3.4.4.
^eHA amino acid variations from the H5N1 clade 2.3.4 viruses are indicated in bolditalic.

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