Table 1 Strains, plasmids and primers used in this study
Strains, plasmids or primers | Characteristics | Source or references |
|---|---|---|
Strains | ||
CH3 | Riemerella anatipestifer serotype 1 strain | [8] |
RA1067 | Tn4351 insertion at M949_RS01915Â gene mutant of Riemerella anatipestifer CH3 | This study |
BW19851(pEP4351) | Plasmid pEP4351 in BW19851, CmR | [8] |
WJ4 | R. anatipestifer wild-type strain, serotype 1 | [8] |
Yb2 | R. anatipestifer wild-type strain, serotype 2 | [8] |
HXb2 | R. anatipestifer wild-type strain, serotype 10 | [8] |
Primers | ||
16S rRNA F | 5′-GAGCGGTAGAGTATCTTCGGATACT-3′ | This study |
16S rRNA R | 5′-AATTCCTTTGAGTTTCAACCTTGCG-3′ | This study |
TN-1 | 5′-GGACCTACCTCATAG-3′ | This study |
IS4351 | 5′-TCAGAGTGAGAGAAAGGG-3′ | This study |
Tn4351-F | 5′-TGGCACCTTTGTGGTTCTTAC-3′ | This study |
Tn4351-R | 5′-GAGAGACAATGTCCCCCTTTC-3′ | This study |
Erm-F | 5′-GCCCGAAATGTTCAAGTTGT-3′ | This study |
Erm-R | 5′-CTTGACAACCACCCGACTTT-3′ | This study |
M949_ RS01915F | 5′-AGTCTGCGTTGATCACCTTT-3′ | This study |
M949_ RS01915R | 5′-AGCAAATACCAACAGAAGGGA-3′ | This study |
M949_ RS01920F | 5′-AACCTCACTATCGGACCAGG-3′ | This study |
M949_ RS01920R | 5′-GCCCGTTCCCAATTATTTGC-3′ | This study |
M949_ RS01910F | 5′-GGAACTGGGATAGACGACCA-3′ | This study |
M949 _RS01910R | 5′-AGAGCAGAGCGTTTACCCTT-3′ | This study |
RA ldh- F | 5′-AGAGGAGCTTATCGGCATCA-3′ | This study |
RA ldh -R | 5′-CTAGGGCTTCTGCCAATCTG-3′ | This study |
RA1067-F | 5′-ATGAATTATTTTAAACTGCT-3′ | This study |
RA1067-R | 5′-TTAGTCTAATTTCTGTATAT-3′ | This study |