Table 4 Descriptive results of the strain data of ten herds and the three batches (1–3) within each herd: prevalence of nPCR (n = 600) positive results, number of different M. hyopneumoniae strains, total number of M. hyopneumoniae strains and number of bronchoalveolar lavage fluid samples obtained with detection of double or triple different strains

nPCR

88 (53/60)

63 (38/60)

87 (52/60)

80 (48/60)

95 (57/60)

83 (50/60)

98 (59/60)

100 (60/60)

63 (38/60)

66 (40/60)

83 (495/600)

 1

100 (20/20)

55 (11/20)

85 (17/20)

95 (19/20)

100 (20/20)

95 (19/20)

95 (19/20)

100 (20/20)

15 (3/20)

5 (1/20)

75 (149/200)

 2

65 (13/20)

55 (11/20)

90 (18/20)

90 (18/20)

85 (17/20)

90 (18/20)

100 (20/20)

100 (20/20)

90 (18/20)

95 (19/20)

86 (172/200)

 3

100 (20/20)

80 (16/20)

85 (17/20)

55 (11/20)

100 (20/20)

65 (13/20)

100 (20/20)

100 (20/20)

85 (17/20)

100 (19/20)

87 (174/200)

Number of different strains

16

6

10

19

18

23

14

15

12

7

135

 1

9

2

5

8

11

13

6

10

2

1

65

 2

9

3

6

9

8

11

9

6

8

4

71

 3

7

1

3

6

10

7

4

5

3

3

49

Number of strains

67

11

66

49

94

46

69

53

23

18

496

 1

25

5

15

18

32

17

21

20

2

1

156

 2

19

4

30

21

29

20

26

19

15

8

191

 3

23

2

21

10

33

9

22

14

6

9

149

BALF double strains

16

0

15

4

36

11

13

6

1

0

102

 1

6

0

1

0

12

5

4

3

0

0

31

 2

5

0

10

3

10

4

5

2

1

0

40

 3

5

0

4

1

14

2

4

1

0

0

31

BALF triple strains

1

0

1

0

1

2

1

0

0

0

6

 1

0

0

0

0

0

0

0

0

0

0

0

 2

1

0

1

0

1

2

1

0

0

0

6

 3

0

0

0

0

0

0

0

0

0

0

0