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Table 5 Mean cycle threshold (Ct) values of cytokine gene transcription in unstimulated endometrial epithelial and stromal cells following 1 and 4 h of incubation

From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

 

Epithelial cells

Stromal cells

1 h

4 h

1 h

4 h

Cytokine

  IL-

33.22

33.91

34.75

34.56

  IL-

29.39

29.67

29.99

30.00

  IL-6

31.48

31.27

33.08

32.84

  TNFα

32.28

32.35

35.52

35.11

  IL-8

24.23

24.53

26.63

27.21

 CXCL10

30.84

31.29

31.43

31.64

  IL-10

33.66

33.98

32.56

32.52

  INFβ

35.15

32.87

32.00

34.21

Housekeeping

 RPL27

20.84

20.95

20.29

20.37

  1. n = 3 uteri and two replicates per uteri. The mean Ct value of the housekeeping gene RPL27 was 20.62 ± 0.61 (Mean ± SD).