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Table 5 Mean cycle threshold (Ct) values of cytokine gene transcription in unstimulated endometrial epithelial and stromal cells following 1 and 4 h of incubation

From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

  Epithelial cells Stromal cells
1 h 4 h 1 h 4 h
Cytokine
  IL- 33.22 33.91 34.75 34.56
  IL- 29.39 29.67 29.99 30.00
  IL-6 31.48 31.27 33.08 32.84
  TNFα 32.28 32.35 35.52 35.11
  IL-8 24.23 24.53 26.63 27.21
 CXCL10 30.84 31.29 31.43 31.64
  IL-10 33.66 33.98 32.56 32.52
  INFβ 35.15 32.87 32.00 34.21
Housekeeping
 RPL27 20.84 20.95 20.29 20.37
  1. n = 3 uteri and two replicates per uteri. The mean Ct value of the housekeeping gene RPL27 was 20.62 ± 0.61 (Mean ± SD).