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Table 2 Primers sequences used for quantitative real time PCR (qRT-PCR)

From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

Gene Accession number Sequence (5′–3′) Amplicon size (bp)
NFKB NM_001003344.1 FW-GGAGGAGACCGGCAGCTTA
RV-GCCGGTGCTATCTGGAAGAA
129
IRF3 XM_005616307.1 FW-TCACCACGCTACACCCTCTG
RV-ATTTCCAGCAGGGCCCTAAG
119
IRF7 XM_005631711.1 FW-GTCGGGGCTCCCCATACTAC
RV-CCCTTCTCGCTGCACGTACT
143
TNF-α NM_001003244.4 FW-CTGCCTCAGCCTCTTCTCCTT
RV-CTGGGCAAGAGGGCTGATTA
133
IL- NM_001037971 FW-GAAGAAGCCCTGCCCACA
RV-AATTATCCGCATCTGTTTTGCAG
104
IL- NM_001003157.2 FW-TTGTGAGTGCCCAAAATGAAGA
RV-CCATGACGCTCCCAAAAGA
109
IL-6 NM_001003301 FW-CTGGCAGGAGATTCCAAGGAT
RV-TCTGCCAGTGCCTCTTTGC
167
IL-8 NM_001003200 FW-TTGCTCTCTTGGCAGCTTTTG
RV-TTTGGGATGGAAAGGTGTGG
122
IL-10 NM_001003077 FW-ACATCAAGAACCACGTGAACTCC
RV-ACTCACTCATGGCTTTGTAGACACC
177
TGF-β1 NM_001003309 FW-AGCCCGAGGCGGACTACTAC
RV-CGGAGCTCTGATGTGTTGAAGA
130
IFN-β NM_001135787.1 FW-GAAGCTCCACTGGCAGAAGG
RW-TGGCCTTCAGGTACTGCACA
137
CXCL10 NM_001010949.1 FW-TCCTGCAAGTCCATCGTGTC
RV-ATTGCTTTCACTAAACTCTTGATGGTC
114
RPL27 NM_001003102 FW-TCGTCAACAAGGATGTCTTCAGAG
RV-TCTTGCCAGTCTTGTACCTCTCCT
96