Table 1 Primer sequences used for RT-PCR
From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli
| Gene | Accession number | Sequence (5′–3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| MyD88 | XM_534223 |
FW-ACTATCGGCTGAAGTTGTGTGTGT RV-TGGTGTAGTCACAGACAGTGATGAA | 58.3 | 276 |
| TRAM | NM_001204337 |
FW-GGGTGTCAGGAAGTCGAAAATA RV-CTGCGTGCAGTATCACAAACTT | 56.8 | 266 |
| TRAF6 | XM_003432322 |
FW-AAACTGTGAAAACAGCTGTGGA RV-CAGTTCATGCAAGAAACCTGTC | 56.1 | 564 |
| TRIF | XM_849573 |
FW-CTTCCAAAGCCCATAGAGGA RV-AAGCGGTGTCTTCTACAGGAA | 55.4 | 487 |
| IRF3 | XM_005616307 |
FW-GGTGCCTACACTCCTGGAAA RV-CTTCATCAGGCACCAAGAGC | 56.0 | 418 |
| NFkB | NM_001003344 |
FW-TGTTTCACTTGGATCCTTTGAC RV-AGATCCCATCCTCACAGTGTTT | 55.4 | 327 |
| IL-1β | NM_001037971 |
FW-CACCAGTGAAATGATGGCTTAC RV-CTCATGTGGAACACCACTTGTT | 56.0 | 453 |
| IL-6 | NM_001003301 |
FW-GTACATCCTCGGCAAAATCTCT RV-GGATGAGGTGAATTGTTGTGTG | 56.0 | 410 |
| IL-8 | NM_001003200 |
FW-TTGGCAGCTTTTGTCCTT RV-GGGCCACTGTCAATCACT | 52.5 | 149 |
| IL-10 | NM_001003077 |
FW-AAGCTGGACAACATACTGCTGA RV-TGTCAAACTCACTCATGGCTTT | 56 | 310 |
| TNF-α | NM_001003244 |
FW-TGACAAGCCAGTAGCTCATGTT RV-CGGCAAAGTCCAGATAGTTAGG | 56 | 410 |
| IL-2 | NM_001003305 |
FW-TTGTCGCAAACAGTGCACCTA RV-CCTGGAGAGTTGGGGGTTCT | 60 | 131 |
| IL-4 | NM_001003159 |
FW- CTCACCAGCACCTTTGTCCA RV- GTCAGCTCCATGCACGAGTC | 60 | 107 |
| IFN-β | NM_001135787 |
FW- CAGTAGATGCATCCTCCAAACA RV- GACTATTGTCCAGGCACAGATG | 55.9 | 493 |
| IFN-γ | NM_001003174 |
FW-GCTGTAACTGTCAGGCCATGTTT RV-TGTTTTGTCACTCTCCTCTCTCCA | 60 | 140 |