Chemokines, anti-inflammatory Interleukin-10 and Interferon-β genes transcription in epithelial and stromal cells after
or LPS cell stimulation. Relative mRNA expression (qRT-PCR) of IL-8 (A), CXCL10 (B), IL-10 (C), INFβ (D) in cultured canine endometrial epithelial and stromal cells in response to hemolytic E. coli (Pyo18), non-hemolytic E. coli (Pyo14) and the isogenic mutant of Pyo18 (Pyo18ΔhlyA). Treatment with 1 μg/mL of LPS was used as a positive control. Data are mean ± SEM (n = 3 uteri with two replicates for all stimuli). Transcription levels (fold increase) are normalized with those of non-stimulated cells. a vs b
p < 0.05 (stromal cells: IL-8); a vs d
p < 0.05 (epithelial cells: IFNβ); c vs d
p < 0.05 (Epithelial cells: IFNβ); b vs c
p < 0.05 (epithelial cells: IFNβ); a vs b
p < 0.01 (stromal cells: IL-10); * vs **p < 0.01 (stromal cells: IL-8); p < 0.01 (1 vs 4 h: IL-8, stromal cells; CXCL10, epithelial cells); p < 0.001 (1 vs 4 h: IFNβ, epithelial cells).