Pro-inflammatory interleukins genes transcription in cultured canine endometrial epithelial and stromal cells after
or LPS cell stimulation. Relative mRNA expression (qRT-PCR) of IL-1β (A), IL-1α (B), IL-6 (C), TNFα (D) in cultured canine endometrial epithelial and stromal cells in response to hemolytic E. coli (Pyo18), non-hemolytic E. coli (Pyo14) and the isogenic mutant of Pyo18 (Pyo18ΔhlyA). Treatment with 1 μg/mL of LPS was used as a positive control. Data are mean ± SEM (n = 3 uteri with two replicates for all stimuli). Transcription levels (fold increase) are normalized with those of non-stimulated cells a vs b
p < 0.05 [(Epithelia cells: IL-1α, IL-6; stromal cells: IL-1β, IL-1α, TNFα]; a vs b
p < 0.01 (stromal cells: IL-6); a vs c
p < 0.05 (stromal cells: TNFα); * vs **p < 0.05 (IL-1β) * vs **p < 0.01 (IL-1α); p < 0.05 (1 vs 4 h: IL-1α, stromal cells); p < 0.01 (1 vs 4 h: IL-6, epithelia cells).