Figure 5

Activation of TLRs signaling pathways evaluated by immunofluorescence. Nuclear detection of NFκB (A–H) and IRF3 (I–P) in endometrial epithelial and stromal cells by immunofluorescence. A–D and I–L epithelial cells [A, I rabbit isotype negative control after 4 h of incubation; B, J unstimulated cells after 4 h of incubation; C, D and K, L stimulation with Pyo18ΔhlyA strain during 1 h (C, K) and 4 h (D, L)]; E–H and M–P stromal cells [E, M rabbit isotype negative control after 1 h of incubation; F, N unstimulated cells after 1 h of incubation; G, H and O, P stimulation with Pyo18ΔhlyA strain during 1 h (G, O) and 4 h (H, P)]. White arrow—nuclear staining.