Figure 1From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli Histology of uteri from bitches diagnosis with pyometra. A–B Histological section of uterine pyometra samples. A non-hemolytic E. coli pyometra evidencing basal glands with myeloid cells infiltration (arrow); B β-hemolytic E. coli pyometra evidencing extensive damage of basal glands (arrow) and metritis (arrow head). Staining by H&E. C–K Immunostaining of calprotectin positive cells (myeloid cells), CD79 αcy positive cells (B lymphocytes) and CD3 positive cells (T lymphocytes) in pyometra uterine sections. Myeloid cells (granulocytes/macrophages) detection: C negative control (staining with mouse isotype); D, E infiltration in apical and basal layer, respectively (cytoplasmic staining pattern). B lymphocytes detection: F negative control (PBS); G, H infiltration in apical and basal layer, respectively (membrane staining pattern). T lymphocytes detection: I negative control (staining with rabbit isotype); J, K infiltration in apical and basal layer (membrane and cytoplasmic staining pattern), respectively.Back to article page