Figure 3

Histopathological scores, IAV antigen quantification by IHC and viral RNA detection by RRT-PCR in the lungs. A Histopathological scores representing lung lesion severity in infected and control (C-) animals. B Immunohistochemical cell quantification of IAV-positive cells in infected and control ferret lungs; results express the mean cell counts with SEM (×20 objective field). C IAV RRT-PCR in the total lung of infected and control animals and D in different lung compartments of infected and control animals. Values represented as inverted Ct values. E Lung viral titration. Values represented as PFU/g. F Nasal swab viral titration. Values represented as PFU/µL. The data expresses the means with SEMS. Statistically significant differences are represented by *p < 0.05.