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Table 1 Median parameters describing influenza viral kinetics for the different challenge groups for virus titre determined by plaque assay

From: Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09

Parameter Unit IN high IN low AERO high AERO low Cont. p value for dose groups testing* p value for route groups testing*
N = 3 N = 2a N = 3 N = 4 N = 6 Cont. vs. high Cont. vs. low Low vs. high Cont. vs. AERO Cont. vs. IN AERO vs. IN
V0 log10 PFU/mL 0.49 [0.48; 0.53] 0.41 [0.34; 0.47] 0.16 [0.15; 0.22] 0.21 [0.07; 0.33] −0.02 [−0.05; 0.01] 0.024 0.024 0.711 0.038 0.009 0.009
s1 log10 PFU/d 3.34 [2.52; 3.52] 0.45 [0.34; 0.56] 1.05 [0.89; 1.18] 0.63 [0.50; 0.77] 0.63 [0.51; 0.69] 0.012 0.755 0.012 0.22 0.22 0.22
Tmax d 1.42 [1.28; 2.00] 5.62 [5.31; 5.93] 3.16 [3.01; 3.25] 5.12 [4.62; 5.52] 5.60 [4.70; 5.72] 0.006 0.589 0.012 0.13 0.13 0.43
s2 log10 PFU/mL/d −0.81 [−0.99; −0.73] −1.41 [−1.92; −0.90] −0.96 [−1.07; −0.83] −1.21 [-1.41; −0.92] −0.68 [−0.73; −0.63] 0.11 0.15 0.33 0.048 0.261 0.837
Predicted peak log10 PFU/mL 4.97 [4.84; 5.09] 3.09 [2.25; 3.93] 3.18 [2.95; 3.72] 3.48 [2.49; 4.40] 3.45 [2.43; 3.96] 0.34 0.98 0.34 0.82 0.47 0.47
Tstart dpi 0.16 [0.15; 0.21] 1.95 [1.31;2.58] 0.80 [0.73; 0.89] 1.33 [0.88; 1.87] 1.69 [1.42; 1.96] 0.018 0.579 0.054 0.32 0.32 0.66
Tend dpi 6.56 [6.27; 6.65] 6.92 [6.48; 7.36] 5.96 [5.56; 5.99] 7.24 [7.00; 7.39] 9.30 [9.11; 9.51] 0.012 0.012 0.024 0.012 0.012 0.831
Predicted dura tion of shedding period d 6.40 [6.06; 6.50] 4.97 [3.90; 6.04] 5.17 [4.67; 5.26] 5.74 [4.86; 6.60] 7.52 [6.57; 8.10] 0.072 0.096 0.839 0.03 0.16 0.70
  1. [Q1–Q3]: represents the interval between the first and third quartile; N: number of pigs; Median computed from the individual parameters.
  2. a2 pigs do not have a virus-positive sample on any occasion after the challenge.
  3. * p value computed from pairwise permtuational t test using 1000 permutation and with p value adjusted for multiple testing using Benjamini & Hochberg method. Cont. is for contact.