SAV RNA kinetics, late co-infection. SAV ΔCt values (SAV Ct–EF1α Ct) in blood and heart from individual co-challenged fish (black dots) assayed by RTqPCR targeting SAV RNA segments. For illustration, SAV ΔCt values from SAV control fish (red x) obtained during the early co-infection, was included in the figure. Red arrow indicate introduction of SAV2 shedders (A, B) or SAV3 shedders (C, D). Weeks post SAV shedder introduction (WPC-SAV) are indicated at the x-axis. N positive individuals (raw Ct < 37.0) of total sampled fish is tabulated under each sub-figure. Asterisk indicates significant (p < 0.05) differences between co-challenged groups and SAV control fish.