PRV RNA kinetics. PRV ΔCt values (PRV Ct–EF1α Ct) in blood and heart from individual co-challenged fish (black and grey dots) and PRV control fish (red x) assayed by RTqPCR targeting PRV RNA segments. Red arrow indicates introduction of SAV shedders early (A, B) or late (C, D). Weeks post PRV shedder introduction (WPC-PRV) are indicated at the x-axis. N positive individuals (raw Ct <37.0) of total sampled fish is tabulated under each sub-figure. Asterisk indicates significant (p < 0.05) differences in PRV RNA levels.