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Table 4 Results of four different algorithms for commercial turkey tissue samples of healthy birds or those infected with Histomonas meleagridis

From: The 60S ribosomal protein L13 is the most preferable reference gene to investigate gene expression in selected organs from turkeys and chickens, in context of different infection models

Healthy turkeys Infected with H. meleagridis
Genes Delta CT BestKeeper© NormFinder GeNorm RefFinder Delta CT BestKeeper© NormFinder GeNorm RefFinder
TFRC 2.39 1.22 0.908 1.333 1 1.9 0.59 1.188 0.868 2
RPL13 2.41 0.91 0.923 1.333 2 1.7 0.64 0.707 0.868 1
RPS7 2.41 1.24 0.918 1.45 3 2.2 1.77 1.623 2.033 6
VIM 3.19 1.36 2.466 1.998 4 2.19 1.19 1.653 1.393 3
HMBS 2.99 2.13 2.148 2.466 5 2.23 1.47 1.687 1.872 7
TBP 3.21 2.06 2.488 2.252 6 2.31 1.8 1.826 2.103 8
RPLP0 3.33 3.21 2.711 2.664 7 2.17 1.43 1.635 1.683 4
HPRT1 3.95 2.78 3.45 2.984 8 2.12 1.67 1.501 1.97 5
  1. Delta CT compares CT value from two reference genes; BestKeeper© calculates standard deviations (SD) based on raw crossing point; NormFinder gives stability value (Sv) by comparing inter and intra group variations; GeNorm value represents the average expression stability (M); RefFinder compares all other algorithms and gives an overall ranking on the basis of geometric mean.