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Table 1 List of mutants screened in the initial round of screening in an intramammary sheep infection model

From: Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep

Group I-15 mutants Group II-17 mutants Group III-19 mutants
Mutant Genes disrupted Mutant Genes disrupted Mutant Genes disrupted
Apo7 Hypothetical Protein (MAG1890) Apo2-12 Hypothetical protein, predicted lipoprotein (MAG1050) 3-20 NADH oxidase (nox)
Apo6 Sugar isomerase (araD) 1-3-0 Hypothetical protein (MAG2810) 13 VpmaX
26 Oligopeptide transporter ATP binding protein (oppF) 15-2 Hypothetical protein, predicted lipoprotein (MAG6200) 14 DNA methylase (MAG1790)
3-15 Elongation factor G (fusA) 170 Hypothetical protein (MAG0110) 137 Hypothetical protein (MAG4460)
6-9-2 Oligopeptide ABC transporter permease (oppC) 3-29 Hypothetical protein/Vpma like predicted lipoprotein (MAG2540) 8-1 Deoxyguaosine kinase (dgk)
6-14 Hexose P transport protein (uhpT) 4-1-0 GTP-dependent nucleic acid-binding protein (engD) 1-2-0 Pentitol posphotransferase
enzyme II (MAG6360)
6-21 Cell division protein (mraZ) 8 + 1-4 ABC transporter ATP-binding protein (MAG5960) 96-2 ATP synthase (atpA)
6-29 Pyruvate dehydrogenase beta subunit (pdhB) 8 + 1-8 Transport protein SGAT (ulaA) 5-1 Hypothetical protein (MAG0250)
6-32 Phosphate acetyl transferase (eutD) 3-4-0 Hypothetical protein (MAG3390) 6-13 Hypothetical protein (MAG1570)
7 + 1-8 Hypothetical protein (MAG4010) 9-28 Conserved hypothetical protein (MAG0390) 7 + 1-3 Conserved hypothetical protein (MAG3650)
9-40 Glycerol ABC transporter permease component (gtsB) 62 Lipoate Protein Ligase A (lplA) 23 Aminopeptidase (MAG5520)
3-1 Phosphoenol-
pyruvate phosphotransferase ( ptsL )
3-1 Phosphoenolpyruvate Phosphotransferase ( ptsL ) 3-1 Phosphoenolpyruvate Phosphotransferase ( ptsL )
8 + 1-5 Ribonuclease H II ( rnhB ) 8 + 1-5 Ribonuclease H II ( rnhB )   
9-31 Alcohol dehydrogenase (adhT) and NADH oxidase (nox)    9-31 Alcohol dehydrogenase (adhT) and NADH oxidase (nox)
6-15 Esterase/lipase ( lip )    6-15 Esterase/lipase ( lip )
   81-1 Oligopeptide ABC transporter permease (oppB) 81-1 Oligopeptide ABC transporter permease (oppB)
   9-8 Dihydrolipoamide dehydrogenase (pdhD) 6-12 Transcriptional regulator (MAG6310)
   212 Type III restriction modification system (MAG1530) San5 Predicted lipoprotein (MAG3120)
   9-16 Conserved hypothetical protein (MAG1450) 3-23 Lipoprotein (MAG6410)
     6-15 VpmaY
  1. Mutants kept common (“watermarks”) between the groups are underlined and those shortlisted for secondary screening showing >95% absence are in italics.