Figure 2

PrP ^Sc accumulation in the brain of Q ₂₂₂ -Tg501 and K ₂₂₂ -Tg516 mice infected with Sheep-Sc M45. Immunohistochemistry with R486 PrP antibody and haematoxylin counterstaining. PrP^Sc remained located to the molecular layer of the cerebellum as amorphous coalescing or plaque-like deposits (arrows) (A, B). Note the absence of PrP^Sc and vacuolation in the hippocampus and dorsal thalamus (C, D). Scale bars are 100 µm (A, B) and 500 µm (C, D).