accumulation in the brain of Q
-Tg501 and K
-Tg516 mice infected with Sheep-Sc M45. Immunohistochemistry with R486 PrP antibody and haematoxylin counterstaining. PrPSc remained located to the molecular layer of the cerebellum as amorphous coalescing or plaque-like deposits (arrows) (A, B). Note the absence of PrPSc and vacuolation in the hippocampus and dorsal thalamus (C, D). Scale bars are 100 µm (A, B) and 500 µm (C, D).