-Tg501 and K
-Tg516 mice inoculated with an atypical scrapie isolate. Immunoblots of brain PrPres detected with 12B2 mAb of atypical scrapie isolates before and after transmission in either Q222-Tg501 or K222-Tg516 mice. Sheep classical scrapie and uninfected sheep brain were included on the right. Similar amounts of 10% brain homogenate were loaded in each lane for comparison. Both Q222-Tg501 and K222-Tg516 mice showed a similar PrPres ladder pattern characteristic of atypical scrapie. Molecular masses (kDa) are shown on the right.