Discriminatory immunoblot of goats with PrP
accumulation in the brain stem. Antibody-binding ratios P4/L42 and the differences of the molecular weights of proteinase K treated, unglycosylated PrPSc bands/form in contrast to the molecular weight of the sheep scrapie control are shown of bovine (R8/09), ovine (486) and caprine (ZG01) BSE, of ovine scrapie (Stdl. 171) and all Cypriot caprine scrapie samples. P4/L42 ratios were calculated as the intensity of the signal strength from P4 versus L42 gained from Western blot. Y-axis: dark grey, P4/L42 antibody binding ratio (AE = arbitrary unit), data obtained by two gel runs; light grey, difference molecular weight of the un-glycosylated form of the PrPSc sample and scrapie control (kDa), data obtained by four to five gel runs.