Schema of immunohistochemical and biochemical tests carried out on selected samples of the Cypriot goats. At first, brain stem samples of all 42 goats were examined by different methods. Subsequently the most likely sites of PrPSc accumulations such as the retropharyngeal lymph node, tonsils, third eye lid, spleen, celiac and mesenteric ganglion complex (CMGC) and rectum of all goats were examined by immunohistochemistry (I). In a second step, samples of the kidney, mammary gland, uterus and, if available, placentomal tissue of the placenta were examined with only taking the goats into account which revealed PrPSc positive results in the tissue samples analysed previously (II). Additionally all positive brain stem samples were examined by discriminatory immunoblot.