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Figure 3 | Veterinary Research

Figure 3

From: Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Figure 3

Separation of the M. haemolytica outer membrane proteins that bind to bovine apolactoferrin (BapoLf) using 2-D gel electrophoresis. A 2-D gel electrophoresis of the M. haemolytica OMP, stained with Coomassie blue. B Overlay of M. haemolytica OMP, from 2-D gel electrophoresis; the nitrocellulose membrane was incubated with HRP-BapoLf. The arrows show the spots of BapoLf binding proteins (A) and their MW and estimated IP (B), which were further identified by mass spectrometry.

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