Figure 2
From: Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1
Bovine lactoferrin (BapoLf and BholoLf) binding to M. haemolytica OMP. A 12% SDS-PAGE of M. haemolytica OMP extracted with sarcosyl, stained with Coomassie blue. B OMP overlay, incubated with BapoLf coupled to horseradish peroxidase (HRP-BapoLf), the arrow shows the 40 kDa BapoLf binding protein; Lane 2, MhF (field strain); lane 3, MhR (reference strain). C Competence between BapoLf and HRP-BholoLf (MhF), OMP transferred were incubated with BapoLf and afterwards with HRP-BholoLf, the arrow shows the 40 kDa BLf binding protein. D OMP overlay (MhF), the membrane transferred was incubated with HRP-lactoferricin B (HRP-LfcinB), in the right line the membrane was incubated with BapoLf (without HRP) and afterwards with HRP-LfcinB; the arrow shows the 40 kDa BLfcin binding protein.