Figure 1
From: Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1
Evaluation of bovine holo-lactoferrin (BholoLf) as a sole iron source in Mannheimia haemolytica strains (MhF and MhR). Moraxella bovis was used as positive control and Actinobacillus pleuropneumoniae as a negative control of use of BholoLf. The bacterial growth was determined by OD595nm, at 24 h of incubation at 37 °C, in agitation (200 rpm) in different conditions: BHI, Brain heart infusion broth; CB, BHI chelated with dipyridyl; CB + FC, chelated BHI plus ferric chloride [80 μM]; CB + BholoLf, chelated BHI plus BholoLf [80 μM of iron]. The results are shown as the mean ± SD, *p < 0.05 versus CB and CB + BholoLf.