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Figure 4 | Veterinary Research

Figure 4

From: Identification of epitopes recognised by mucosal CD4+ T-cell populations from cattle experimentally colonised with Escherichia coli O157:H7

Figure 4

Location and conservation of bovine CD4 + T-cell Intimin epitopes after experimental colonisation with EHEC O157:H7. A Schematic of EHEC Intimin-γ, labelled with structural domain coordinates (Uniprot accession: P43261). Location of CD4+ T-cell epitopes is indicated in red. B Sequence alignment of CD4+ T-cell epitopes against the parental EHEC O157:H7 Intimin-γ sequence (accession BAB37982.1) and an Intimin consensus sequence. Conservation of this consensus is indicated on a scale of (*)-0, where * = 100% identity, 9 is highly conserved, down to 0, which is not conserved. Structural domains shown in A are also highlighted in this alignment. The consensus sequence was generated by a MUSCLE [32] multiple sequence alignment of representative Intimin sequences from EHEC of public health concern: O157:H7 (γ, accession BAB37982.1); O145:H28 (γ, accession AHY73165.1); O26:H11 (β, accession BAI28422.1); O103:H2 (ε, accession BAI32332.1); O121:H19 (ε, accession KDV53260.1); O45:H2 (ε, accession EZA22403.1); O111:H- (τ, accession BAI37528.1) and a prototypical EPEC O127:H6 comparator (α, accession YP_002331401.1). Intimin types are indicated in brackets. Conservation levels of this consensus sequence were generated using the PRALINE consistency output [33].

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