Figure 3

IFN-γ production by rectal CD4 ⁺ T-cells of EHEC O157 colonised calves in response to individual bacterial peptides. CD4⁺ T-cell lines prepared from the rectal lymph nodes of animals challenged with EHEC O157:H7 were stimulated with individual peptides from the top eight positive pools for each animal in Table 1 and subsequent IFN-γ release quantified by ELISA. Each panel represents results from a single animal. Peptides are ordered on the Y axis by the amount of IFN-γ produced. Peptide names are given for positive peptides only. Solid lines denote the mean of four negative control wells. Dotted lines denote three standard deviations from the negative control as detailed in the "Materials and methods" section. Positive peptides are shown in the inset next to each graph.