Figure 2

IFN-γ production by rectal CD4 ⁺ T-cells of EHEC O157 colonised calves in response to pooled bacterial peptides. CD4⁺ T-cell lines prepared from the rectal lymph nodes of animals challenged with EHEC O157:H7 were stimulated with pools of peptides from 16 EHEC O157:H7 proteins and subsequent IFN-γ release quantified by ELISA. Each panel represents results from a single animal. Peptide pools are ordered on the Y axis by the amount of IFN-γ produced. Peptide pool names are given for positive pools only. Solid lines denote the mean of two negative control wells. Dotted lines denote three standard deviations from the negative control as detailed in the "Materials and methods" section. Positive pools are shown in the inset next to each graph.