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Figure 1 | Veterinary Research

Figure 1

From: Identification of epitopes recognised by mucosal CD4+ T-cell populations from cattle experimentally colonised with Escherichia coli O157:H7

Figure 1

Experimental approach. A Graphical representation of APC and CD4+ T-cell line generation. B, C Immunofluorescent staining of T. annulata immortalised PBMC. Nuclei stained using DAPI (blue) and macroschizonts stained using monoclonal antibody 1C12 (green). D Flow cytometric analysis following staining of T. annulata immortalised PBMC for MHCII, CD80 and CD86. Filled histograms denote stained samples, open histograms denote the no primary control.

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Veterinary Research

ISSN: 1297-9716