Experimental approach. A Graphical representation of APC and CD4+ T-cell line generation. B, C Immunofluorescent staining of T. annulata immortalised PBMC. Nuclei stained using DAPI (blue) and macroschizonts stained using monoclonal antibody 1C12 (green). D Flow cytometric analysis following staining of T. annulata immortalised PBMC for MHCII, CD80 and CD86. Filled histograms denote stained samples, open histograms denote the no primary control.