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Table 1 Strains and plasmids used in the study

From: Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse

Strains and plasmids Characteristics Source
Brucella abortus
S2308 Wild type strain; Smooth phenotype ATCC
pyk pyk gene deletion mutant strain; Smooth phenotype This study
pyk(Pyk-3 × Flag) Cmr; complementation strain; ∆pyk carrying the complementation plasmid pBBR-pyk-3 × Flag; Smooth phenotype This study
Escherichia coli
DH5α F φ80lacZ∆M15 ∆(lacZYA-argF)U169 recA1 endA1 hsdR17(r k , m +k ) phoA supE44 thi-1 gyrA96 relA1 λ Invitrogen
Plasmids
pBBR1MCS1 Cmr; Broad-host-range cloning vector [17]
pSC Ampr; pUC19 plasmid containing SacB gene [16]
pSC-∆pyk Ampr; pSC plasmid containing the ∆pyk fragment; used to construct deletion strain This study
p3 × Flag-CMV-14 Ampr; eukaryotic expression plasmid Sigma-Aldrich
pBBR-pyk-3 × Flag Cmr; pBBR1MCS1 containing the pyk gene flanked by its upstream and downstream regions containing a C-terminal 3 × flag tag. This study