mutant was not able to efficiently exclude lysosome-associated membrane protein-1 within RAW264.7 cells. A Representative images of LAMP-1-positive and -negative BCVs. B Determination of the LAMP-1 positive BCVs at 4 and 24 h post-infection of the S2308 strain and the pyk mutant. The percentage of positive BCVs per 100 random BCVs is shown. ***p ≤ 0.001. ns: no significant difference.