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Figure 3 | Veterinary Research

Figure 3

From: Chicken interferome: avian interferon-stimulated genes identified by microarray and RNA-seq of primary chick embryo fibroblasts treated with a chicken type I interferon (IFN-α)

Figure 3

qRT-PCR validation and kinetic analysis of ISG expression. (A) Validation of RNA-seq and Microarray ISGs by qRT-PCR of CEF treated with recombinant chicken IFN1 (1000 units, 6 h). (B) Kinetics of expression of selected ISGs assayed by qRT-PCR following treatment of CEF with recombinant chicken IFN1 (1000 units) for 45, 90, 180 or 360 min. ISGs showing similar kinetic expression profiles are paired vertically.

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Veterinary Research

ISSN: 1297-9716