Figure 6
Effect of baicalin on the activation of NLRP3 inflammasome signaling pathway induced by H. parasuis in peripheral blood monocytes. A–C 1 × 107 of cells were pre-treated with baicalin of 25, 50, 100 μg/mL for 2 h. Then 1 × 107 CFU/mL H. parasuis were added into the wells and co-incubated for 3, 6 h respectively. Hence the cells were collected and total cellular RNA was extracted and was reverse-transcribed to cDNA. D The monocyte cells of 3 × 107 were pretreated with baicalin (25, 50, 100 μg/mL) for 2 h, then 3.0 × 107 CFU/mL H. parasuis was added and co-cultured with 5 h, the cells were collected and then the cell protein was extracted for detection of levels of cleaved caspase-1. The white bar represents the control group. The black bar represents the model group. The twilled bars represent the baicalin-pretreated groups. ## p < 0.01 vs. control. **indicates significance at p < 0.01.