Figure 4
Effect of baicalin on the production of reactive oxygen species (ROS) and cell apoptosis induced by H. parasuis in peripheral blood monocytes. A 1 × 106 of cells were seeded into 24-well plates and treated with various concentrations of baicalin (25, 50, 100 μg/mL) for 2 h. Then 1 × 106 CFU/mL H. parasuis were added into the wells and incubated for 3, 6 h respectively. Hence the incubations were washed three times with PBS and stained with 10 μM DCFH-DA and 5 μM DHE for 30 min, respectively. The fluorescence intensities were observed by Fluorescence microscope. B 1 × 106 of cells were seeded into 24-well plates and treated with various concentrations of baicalin (25, 50, 100 μg/mL) for 2 h. Then 1 × 106 CFU/mL H. parasuis were added into the wells and incubated for 3, 6 h respectively. Hence the incubations were washed three times with PBS and stained with Annexin V-FITC and Propidium Iodide, respectively. The fluorescence intensities were observed by Fluorescence microscope. The white bar represents the control group. The black bar represents the model group. The twilled bars represent the baicalin-pretreated groups. ## p < 0.01 vs. control. **indicates significance at p < 0.01.