Effect of baicalin on the reduction of proinflammatory cytokines induced by
in peripheral blood monocytes.
A–H 5 × 105 cells were seeded into 24-well plates and pre-treated with a final concentration of baicalin of 25, 50, 100 μg/mL for 2 h. Then 1 × 106 CFU/mL H. parasuis were added into the wells and incubated for 6 h respectively. Hence the supernatants from the cells were collected and centrifuged at 400 × g for 15 min under 4 °C. Cytokine concentration in the cell culture supernatants was measured by ELISA assays. I–O For RNA determination, 1 × 107 of cells were seeded into 24-well plates and pre-treated with baicalin at 25, 50, 100 μg/mL for 2 h. Then 1 × 107 CFU/mL H. parasuis were added into the wells and co-incubated for 3, 6 h respectively. Hence the cells were collected, then the total cellular RNA was extracted and was reverse-transcribed to cDNA. Individual transcripts in each sample were repeated three times and β-actin was used as the internal control. The white bar represents the control group. The black bar represents the model group. The twilled bars represent the baicalin-pretreated groups. #
p < 0.01 vs. control. **indicates significance at p < 0.01, *indicates significance at p < 0.05.