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Figure 3 | Veterinary Research

Figure 3

From: Baicalin suppresses NLRP3 inflammasome and nuclear factor-kappa B (NF-κB) signaling during Haemophilus parasuis infection

Figure 3

Effect of baicalin on the reduction of proinflammatory cytokines induced by H. parasuis in peripheral blood monocytes. AH 5 × 105 cells were seeded into 24-well plates and pre-treated with a final concentration of baicalin of 25, 50, 100 μg/mL for 2 h. Then 1 × 106 CFU/mL H. parasuis were added into the wells and incubated for 6 h respectively. Hence the supernatants from the cells were collected and centrifuged at 400 × g for 15 min under 4 °C. Cytokine concentration in the cell culture supernatants was measured by ELISA assays. IO For RNA determination, 1 × 107 of cells were seeded into 24-well plates and pre-treated with baicalin at 25, 50, 100 μg/mL for 2 h. Then 1 × 107 CFU/mL H. parasuis were added into the wells and co-incubated for 3, 6 h respectively. Hence the cells were collected, then the total cellular RNA was extracted and was reverse-transcribed to cDNA. Individual transcripts in each sample were repeated three times and β-actin was used as the internal control. The white bar represents the control group. The black bar represents the model group. The twilled bars represent the baicalin-pretreated groups. # p < 0.01 vs. control. **indicates significance at p < 0.01, *indicates significance at p < 0.05.

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