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Table 1 Primers used in this study for RT-PCR and real time qRT-PCR

From: Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways

Primer name

Sequence (5′-3′)

References

Forward primer

Reverse primer

ATMUV E

CCTACTGACACTGGGCATGG

AGGCAACCATCCTTTGTGCT

 

Chicken β-actin

GCCAACAGAGAGAAGATGACAC

GTAACACCATCACCAGAGTCCA

[41]

Chicken IFN-α

ATGCCACCTTCTCTCACGAC

AGGCGCTGTAATCGTTGTCT

[41]

Chicken IFN-β

ACCAGGATGCCAACTTCT

TCACTGGGTGTTGAGACG

 

Chicken IFN-λ

AGGATGAAGGAGCAGTTTGA

CCAGAGGGCTGATGTGAA

 

Chicken TLR1

GGCAGTGGACGCAGACAAA

GTAGGAAATGAAGGCGTGGAA

[41]

Chicken TLR2

CTGAAGCCACAGACATTCCTAAC

CTTGTACCCAACGACCACCA

[41]

Chicken TLR3

GCAACACTTCATTGAATAGCCTTGAT

TTCAGTATAAGGCCAAACAGATTTCC

[41]

Chicken TLR4

GGCAAAAAATGGAATCACGA

CTGGAGGAAGGCAATCATCA

[41]

Chicken TLR5

TCAAAGATGGGTGGTGTGTAGAA

ACTGACGTTCCTTTGCACTTTTT

[41]

Chicken TLR7

ATGCTGTTATCAGGACGTTGGTT

CCTTGAGGCGACGGTCACT

[41]

Chicken TLR15

AACATCTACATCCGTAACCCG

TTAGCACCAGAACGACAAGG

[41]

Chicken TLR21

CAAGAAGCAGCGGGAGAAG

TCAGGATGCGGTTAAAGCG

[41]

Chicken MAD5

TGAAGGCAAAGAGAGATCAGCGTAAGA

CATATCAATTGTGGCAATTCTTGCACAGGA

[40]

Chicken IPS-1

GCAGTTTGATGCAGAGCAGAAGCA

AGGCTTCAAGGAGGTGTCACAGAA

[40]

Chicken Mx1

TTCACGTCAATGTCCCAGCTTTGC

ATTGCTCAGGCGTTTACTTGCTCC

[40]

Chicken OASL

GCAGAAGAACTTTGTGAAGTGGCG

TCGGCTTCAACATCTCCTTGTACC

[40]

Human GAPDH

AGAAGGCTGGGGCTCATTTG

AGGGGCCATCCACAGTCTTC

[39]

Human IFN-β

GCTCTCCTGTTGTGCTTCTCCAC

CAATAGTCTCATTCCAGCCAGTGC

 

Human IL-28A/B

AGCTGCAGGCCTTTAAGAGG

TCCAGAACCTTCAGCGTCAG

[39]

Human IL-29

CCAAGCCCACCACAACTGGG

TCTGGTGCAGGGTGTGAAGG

 

Human TLR3

TCACTTGCTCATTCTCCCTT

GACTCTCCATTCCTGGC

 

Human MAD5

CTGCTGCAGAAAACAATGGA

TGGCTGAACTGTGGTTGAAA

 

Human IRF3

CTGGGGCCCTTCATTGTAGA

TAGGCCTTGTACTGGTCGGA

 

Human IRF7

CGAGACGAAACTTCCCGTCC

GCTGATCTCTCCAAGGAGCC

 

Human IPS-1

TTCTCCTCCTCATCCCCTGG

GGATGGTGCTGGATTGGTGA

 

Human Mx1

GACATTCGGCTGTTTACC

GCGGTTCTGTGGAGGTTA

 

Human OASL

CCCTGAGGTCTATGTGAGC

GTGAAGCCTTCGTCCAAC

 

Human OAS1

AGAGACTTCCTGAAGCAGCG

GAGCTCCAGGGCATACTGAG

 

Human IFITM3

TGGCCAGCCCCCCAACTAT

CATAGGCCTGGAAGATCAG

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