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Figure 8 | Veterinary Research

Figure 8

From: Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways

Figure 8

Pretreatment of host cells with IFN significantly impairs replication of ATMUV. A CEF cells were incubated with avian IFN (1000 IU/mL) for the indicated time before ATMUV infection at a MOI of 0.1. Quantitative real-time PCR were performed to examine expression of ATMUV envelop gene. B 293T cells were pretreated with human IFN-β (500 IU/mL) for the indicated time before infection with ATMUV at a MOI of 0.1. Expression of ATMUV envelop gene was examined by quantitative real-time PCR. The viral mRNA level in control cells (no IFN pretreatment cells) after ATMUV infection was set to 100. The virus suppression efficiency in IFN pretreatment cells was compared to the control. The average results from three independent experiments are plotted. The error bars represent the S.E. *P < 0.05.

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