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Figure 7 | Veterinary Research

Figure 7

From: Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways

Figure 7

IRF3, IRF7 and NF-κB activation are required for efficient expression of IFN induced by ATMUV. 293T cells stably expressing specific shRNA targeting IRF3, IRF7 or luciferase control were infected with or without ATMUV for 36 h. Quantitative real-time PCR were performed to measure the interference efficiency of IRF3, IRF7 (A) and the production of IFN-β, IL-28A/B and IL-29 (B, C). CEF (D) and 293T (E) cells infected with ATMUV for the indicated times were harvested and protein expression of IκB-α was examined by Western blotting. F 293T cells were treated with BAY11-7082 (5 µM) or DMSO for 3 h, followed by ATMUV infection for 36 h infection. The cells were harvested and the mRNA levels of IFN-β, IL-28A/B and IL-29 were analyzed by quantitative real-time PCR. Shown are representatives of three independent experiments with similar results. The mRNA level of positive control cells was set to 100. The average results from three independent experiments are plotted. The error bars represent the S.E. *P < 0.05, **P < 0.01.

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