Figure 5

ATMUV infection triggers innate immune response via MDA5 and TLR3-dependent signaling pathways. 293T cells stably expressing shRNA specifically targeting either TLR3, MDA5 or luciferase control were infected with or without ATMUV for 36 h. Quantitative real-time PCR were then performed to determine the interference efficiency of TLR3, MDA5 (A) and examine the IFN expression in 293T cells stably expressing shRNA targeting TLR3 or MDA5 (B, C). D and E 100 nM TLR3-siRNA or NC-siRNA was transfected into MDA5-knockdown or luciferase-knockdown 293T cell lines, interference efficiency of TLR3, MDA5 and IFN expression were analysed by quantitative real-time PCR. The mRNA level in ATMUV infected 293T cells expressing luc-shRNA control was set to 100. The average levels from three independent experiments are plotted. The error bars represent the S.E. *P < 0.05, **P < 0.01.