Figure 2
The chicken innate immune response was induced by ATMUV infection. Each young SPF chick was challenged by intramuscular inoculation with 4.0 × 105 EID50 of ATMUV in a volume of 0.4 mL. A–D The spleen tissues of mock and ATMUV infected chicks were collected at the time indicated for examination of IFN-β (A), IFN-λ (B) and ISG (C, D) mRNA expressions using quantitative real-time PCR. The average levels from three independent experiments are plotted. The error bars represent the S.E. *P < 0.05, **P < 0.01.