Figure 2

Serology, RNA detection and cross neutralization results following single infections with BTV1, BTV15 and BTV8. A Results of RTqPCR and SNT in V and NV groups of calves, after BTV1 and BTV15 single infection challenges compared with BTV8 first challenge in NV group. Dashed lines represent SNT results. B cELISA % of negativity following BTV1 and BTV15 single infection, BTV8 vaccination in V group and first BTV8 challenge in NV group. Dotted line represents positivity threshold, with % of negativity <35 considered as positive. C Cross neutralization results, with BTV1, BTV8 and BTV15 compared to the respective heterologous immune serum. Results are expressed as a percentage of the highest homologous neutralization titre. *P < 0.05; **P < 0.01.